Using a Microwave to Prepare C-Fern® Powdered Medium Makes C-Fern® Even Easier to Use!
C-Fern® users who do not have access to an autoclave can successfully use a microwave to prepare Basic C-Fern® Powdered Medium.
Although the lower temperatures and shorter times of microwaving cannot guarantee sterility to the degree that autoclaving does, this method has been repeatedly used to successfully prepare contaminant-free medium.
Each packet of powdered medium contains the nutrients for 1 L of medium.
While stirring, add the contents of the packet to 1 L of deionized or distilled water. Rinse out the packet to be sure to get all the powder.
Add 10 g Difco Bacto® Agar.
Adjust the pH to 6.0 with 1.0 N NaOH.
Process the solution in a microwave unit as specified in the following table. You may need to compensate for microwave units of different powers by adjusting the suggested times. Plates should be poured ¾ full.
Be sure to use caution when heating and handling! Wear glasses, use gloves and do not leave microwave unit unattended!
|For 1 L of medium prepared in a 2 L Erlenmeyer flask1|
(for 1000W output unit)
|State of Solution2||Post-microwave
|1||5 minutes||hot||remove, swirl|
|2||1 minute||boiling||remove, swirl|
|3||15 seconds||boiling||remove, swirl|
|4||15 seconds||boiling||remove, swirl|
|5||15 seconds||boiling||swirl to mix and pour Petri dishes|
1Be sure to use a vessel at least twice the volume of the medium.