The Effect of Sucrose Concentration On Sporophyte Development in Ceratopteris
Heather Forbes (Instructors: Laurie Brown and Marilyn Garne, West Iredell High School, Statesville NC)
This project involved an in-depth study of the life cycle and physiology of an unusual type of fern known as C-Fern of the genus Ceratopteris. Particular concern was given to the designing of methods to successfully culture and maintain this delicate organism. The focus then turned to study the effects of sucrose in the media on sporophyte development of Ceratopteris. I designed and built a growth chamber and a sterile transfer/study chamber to lessen the chance of contamination and create optimal growth conditions. Both apparatuses were made from scratch with homemade materials. Spores were obtained and sown in sterile agar plates. Germination was shortly observed with subsequent development of gametophytes. At maturity, fertilization was accomplished by adding droplets of sterile water directly onto the gametophyte. Sporophyte development was soon obvious. Sterile nutrient plates were prepared with 1.5%, 3.0%, and no sucrose. Sporophytes were transferred and grown on each type of media. Sporophyte growth was measured and recorded. Results suggest that sucrose is not a desirable environment for C-Fern. Plans are underway to continue genetic studies of mutant forms of C-Fern.